Fig. 5

OA-RD17 suppressed inflammatory factor expression and activated the MAPK signaling pathway to promote keratinocyte proliferation and migration. A, B ELISA analysis of effects of OA-RD17 on inflammatory factors (IL-6 and TNF-α) release in keratinocytes after LPS stimulation. C Representative image of MAPK signaling pathway activation in keratinocytes after 24 h of OA-RD17 treatment, determined by western blotting. D Quantification of P38, ERK, and JNK protein phosphorylation. E–G RT-qPCR analysis of Ki67, CDK2, and Cyclin A1 mRNA expression in OA-RD17-treated keratinocytes. H Changes in pro-proliferation activity of OA-RD17 in keratinocytes after application of MAPK signaling pathway inhibitors. I Representative images of effects of OA-RD17 on keratinocyte scratch repair after application of MAPK signaling pathway inhibitors. J Quantification of effects of OA-RD17 on keratinocyte scratch repair rate after application of MAPK signaling pathway inhibitors. All data are expressed as mean ± SEM from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001