Fig. 6

OA-RD17 promoted keratinocyte proliferation and migration via MAPK signaling pathway activation by TLR4. A Representative image of molecular docking of OA-RD17 with TLR4. B Representative images of immunofluorescence for co-localization of TLR4 and OA-RD17 in keratinocytes after treatment with FITC-labeled OA-RD17 for 1 h; green fluorescence for FITC-labeled OA-RD17, red fluorescence for TLR4, and DAPI blue for nuclei; the white arrows indicated the binding of TLR4 and OA-RD17; scale bar 50 µm. C Changes in pro-proliferative activity of OA-RD17 on keratinocytes 24 h after TLR4 inhibition. D Representative images of the effects of OA-RD17 on keratinocyte scratch healing after TLR4 inhibitor treatment. E Quantification of pro-keratinocyte scratch repair by OA-RD17 after TLR4 inhibition. F Western blot analysis of MAPK signaling pathway activation in OA-RD17-treated keratinocytes 24 h after TLR4 inhibition. G–I Quantification of OA-RD17-activated MAPK signaling pathway (P38, ERK, and JNK phosphorylation) in keratinocytes. All data are expressed as mean ± SEM from three independent experiments, ns, no significance, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001