Fig. 7

MiR-632 promoted β-catenin expression by targeting GSK3β and activated the Wnt/β-catenin signaling pathway to promote keratinocyte proliferation and migration. A Changes in miR-632 expression in OA-RD17-treated keratinocytes after application of MAPK signaling pathway inhibitors for 24 h, detected by RT-qPCR. B Prediction of binding sites of miR-632 to GSK3β. C Luciferase reporter assay of miR-632 targeting GSK3β. D Effects of miR-632 up-regulation on Wnt/β-catenin signaling pathway, detected by western blotting. E Up-regulation of miR-632 expression significantly inhibited expression of GSK3β protein. F–J Up-regulation of miR-632 expression significantly promoted expression of β-catenin, nuclear accumulation of β-catenin, Vimentin, Cyclin D1, and c-MYC proteins. All data are expressed as mean ± SEM from three independent experiments, ns, no significance, ***P < 0.001, and ****P < 0.0001