Fig. 8

Up-regulation of miR-632 expression activated Wnt/β-catenin signaling pathway to promote skin wound regeneration in rats. A Schematic of full-thickness skin wound in SD rats and subcutaneous injection of miR-632 agomir on postoperative days 0 and 4, with subsequent study manipulations. B, C Representative images of wound repair in SD rats treated with miR-632 agomir for 9 days and quantification of wound repair rate. D Representative H&E staining of wound tissue after 9 days of miR-632 agomir treatment in SD rats; Yellow dotted lines represent areas of neoepithelium; Es: eschar; NE: neoepithelium; GT: regenerated granulation tissue; Yellow arrows represent quantified areas of regenerated granulation tissue; scale bar 200 μm. E, F Quantification of epidermal regeneration thickness and regenerated granulation tissue in wound area. G Western blot analysis of Wnt/β-catenin signaling pathway activation in wound region of SD rats after 9 days of miR-632 agomir treatment. H–L Quantification of GSK3β, β-catenin, Vimentin, Cyclin D1, and c-MYC expression after up-regulation of miR-632. All data are expressed as mean ± SEM from three independent experiments, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001