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Fig. 1 | Cellular & Molecular Biology Letters

Fig. 1

From: Exogenous and endogenous dsRNAs perceived by plant Dicer-like 4 protein in the RNAi-depleted cellular context

Fig. 1

Expression in S. cerevisiae and in vitro functionality of recombinant DCL4. A Western blot analysis of whole cell lysates, separated in an 8% polyacrylamide gel, showing HA-epitope-tagged DCL4 expression in S. cerevisiae after 24 h growth at 21°C, 25°C and 30°C, respectively. B Flow chart used for DCL4 enrichment and in vitro functionality. C Spectrophotometric curve of the DCL4-enriched yeast fraction (DEYF). D Western blot analysis of DEYF: PageRuler Prestained protein Ladder (M), total yeast lysate (lanes 1–3), eluted fractions from anti-HA agarose-conjugated columns (lanes 4–6), DEYF fraction > 50 kDa (lane 7), flow-through of the concentration step < 50 kDa (lane 8), HA peptide in TBS-Tween (lane 9). E In vitro functionality of DEYF in processing single-stranded and double-stranded defective-interfering (DI) RNA transcripts of Tomato bushy stunt virus. Arrows highlight the sRNAs

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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