Fig. 5

Impact of HA-DCL4 expression in Saccharomyces cerevisiae on annotated and putative antisense transcripts. Representation of the genomic organization and distribution of 21- and 22-nt DCL4-derived sRNAs of FMP49/HGV1 (A), IMO32/NAG1 (B), CYC1/UTR1 (C), and ICL1 (E). sRNAs of positive and negative strands are red and blue, respectively. Bars in dark gray are the genes under analysis. Due to the large amount of reads mapped to this locus, positive and negative sRNA reads are represented with a coverage plot, in C, and the additional green coverage plot represents RNA transcript from paired ends NGS. D Heat map representing the expression of selected putative antisense RNA loci upon induction of HA-DCL4 expression. Significance: **adjusted p-value < 0.05 and abs(log2(FC)) > 0.585, *adjusted p-value < 0.05