Fig. 2
From: γ-2 and GSG1L bind with comparable affinities to the tetrameric GluA1 core
γ-2 displaces GSG1L from binding sites. A, C Coexpression of GluA1-tdCherry and GSG1L-GFP yields mainly yellow spots, and a few green and red spots. B, D When unlabeled γ-2 is coexpressed in large excess with GluA1-tdCherry and GSG1L-GFP, it displaces GSG1L-GFP from binding sites, and mainly green and red spots remain. Scale bars 2 µm. The intensities at the spot centers in C and D are moved to the saturated range to better visualize the green/red overlap, or lack thereof. E Colocalization fraction (mean ± s.d.) when GluA1-tdCherry is coexpressed with GSG1L-GFP or γ-2-GFP, with or without unlabeled γ-2 or GSG1L, respectively