Fig. 3
From: γ-2 and GSG1L bind with comparable affinities to the tetrameric GluA1 core
The anti-GFP nanobody binds GFP but not mNeonGreen. A A647-Nb injected into oocytes expressing GluA1-GFP shows strong colocalization with GluA1-GFP. B A647-Nb does not label the extracellular GFP of GFP-GluA1. Weak red spots in the background are from intracellular A647 molecules. C A647-Nb binds to the non-fluorescent GFP(Y66L) of GFP-GluA1-GFP(Y66L). D A647-Nb does not label intracellular mNeonGreen of GluA1-mNeonGreen. E Photobleaching steps of GluA1-mNeonGreen. F A representative distribution of mNeonGreen photobleaching steps with a binomial fit. Scale bars 2 µm