Fig. 5
From: γ-2 and GSG1L bind with comparable affinities to the tetrameric GluA1 core
Determination of binding constants Kγ and KG for γ-2 and GSG1L to the receptor core. A State diagram with transition rates for an AMPA receptor core (A) with different numbers of GSG1L (G) and γ-2 (γ) bound (e.g., AG1γ2 means “core with one GSG1L and two γ-2 bound”). B Fit of the state model to the experimental spot densities for an individual representative experiment. FR, G, and R refer to the presence of far-red fluorescence [A647-Nb bound to GluA1-GFP(Y66L)], the number of green bleaching steps (GSG1L-mNeonGreen), and the presence of red fluorescence (γ-2-mCherry), respectively. C Mean values from eight experiments and results of a common fit (maximum likelihood values and 68% confidence intervals) for the binding constants Kγ and KG