Fig. 2

The verification of transfection efficiency and the changes in ovarian cancer cell migration, invasion and clonogenesis after transfection. RT-qPCR was used to verify EFEMP2 mRNA expression in (a) ES-2 cells after shRNA transfection, and (b) OVCAR-3 cells after over-expression transfection. c ICC verified EFEMP2 protein expression in ES-2, ES-2 shRNA1, ES-2 shRNA2, OVCAR-3, and OVCAR-3 EX cells. d The change in the migration ability of the cells after shRNA transfection and over-expression transfection, and (e) the histogram displayed the number of cells passing through the chamber membrane. f The change in the invasion ability of the cells after shRNA transfection and over-expression transfection, and (g) the histogram displayed the number of cells passing through the chamber membrane coated with Matrigel. h Plate clone assays were used to detect the clonogenic ability of ES-2, ES-2 shRNA1, ES-2 shRNA2, OVCAR-3, and OVCAR-3 EX cells, and i the histogram indicated the number of clones formed. Magnification × 200, *p < 0.05, **p < 0.01, or***P < 0.001