Fig. 4

The downstream pathway of EFEMP2 was detected by phosphorylation pathway profiling array and the relationship between EFEMP2 and PD-L1 expression. ES-2 and ES-2 shRNA1 cell extracts were subjected to the phosphorylation pathway profiling array, (a) the membranes were scanned and (b) the histogram shows the normalization of target genes by positive control points. c Different EFEMP2 expression cells were used for proteomic analysis and the volcano map of differentially expressed genes was drawn. d A bubble chart showed the enrichment analysis of the KEGG pathway of differentially expressed genes. RT-qPCR was used to verify PD-L1 mRNA expression in e ES-2, ES-2 NC, ES-2 shRNA1, and ES-2 shRNA2 cells after EFEMP2 down-regulation, and (f) OVCAR-3, OVCAR-3 NC, and OVCAR-3 EX cells after EFEMP2 over-expression. g Western blot was used to detect the protein expression of PD-L1 in ES-2, ES-2 NC, ES-2 shRNA1, ES-2 shRNA2, OVCAR-3, OVCAR-3 NC, and OVCAR-3 EX cells, respectively. *p < 0.05, **p < 0.01, or***P < 0.001