Fig. 7

Acquisition of PD-L1 stable down- and over-expression cell models and the effects of PD-L1 up- or down- regulation on the migration, invasion and clonogenicity of ovarian cancer cells in vitro and the growth of subcutaneous graft tumor in vivo. Western blot (a) and RT-qPCR (b) verified the transfection effects, and PD-L1 expression was significantly decreased in PD-L1 shRNA transfected group and EFEMP2 cDNA + PD-L1 shRNA transfected group, meanwhile the expression of PD-L1 was remarkably increased in PD-L1 cDNA transfected group and EFEMP2 shRNA + PD-L1 cDNA transfected group. c Changes in cell migration and invasion ability after transfection with PD-L1 shRNA or cDNA, and (d) the histogram displayed the number of cells passing through the chamber membrane in migration and invasion assay. e Plate clone assays were used to detect the change of cloning ability before and after transfection of PD-L1 shRNA or cDNA, and (f) the histogram indicated the number of clones formed in the down-expression group and overexpression group. g The images of subcutaneous graft tumors after inoculation in PD-L1 down-expression group and PD-L1 overexpression group. h The graph illustrated the growth rate and size of tumors in nude mice after PD-L1 shRNA or cDNA transfection. Magnification × 200. *p < 0.05, **p < 0.01, or ***p < 0.001