Fig. 8

The important role of PD-L1 in EFEMP2 promoting the invasion of ovarian cancer cells. a After transwell assay, in OVCAR-3 cells co-transfected with EFEMP2 cDNA and PD-L1 shRNA, the down-regulation of PD-L1 significantly reversed the increase in invasion and mobility caused by overexpression of EFEMP2, conversely, overexpression of PD-L1 in ES-2 cells co-transfected with EFEMP2 shRNA and PD-L1 cDNA enhanced the invasion and mobility inhibited by EFEMP2 knockdown. b the histogram displayed the number of cells passing through the chamber membrane in migration and invasion assay. c By plate cloning formation assay, the down-regulation of PD-L1 significantly inhibited the increase of cell cloning induced by EFEMP2 overexpression, on the contrary, overexpression of PD-L1 significantly reversed the reduction in the number of cell clones caused by EFEMP2 knockdown. d the histogram indicated the number of clones formed. e The images of subcutaneous graft tumors after inoculation in the PD-L1 down-regulated and up-regulated groups. f The graph illustrated the growth rate and size of tumors in nude mice after PD-L1 shRNA or cDNA transfection. Magnification × 200. *p < 0.05, **p < 0.01, or ***p < 0.001