Fig. 2

Surface plasmon resonance (SPR) revealed the specificity of C2 fusion proteins to PS in artificial hybrid bilayer lipid membranes (hBLM). a Schematic representation of SPR: the monochromatic light is reflected on a gold surface. At a certain angle, where the surface plasmons are excited, the reflected light has a minimum, which is continuously measured. The angle changes upon protein binding on the lipid surface. b–f, i Representative SPR sensorgrams of C2, C2-mKate, C2m2-mKate, C2-SNAP, C2m2-SNAP binding to the membranes of different composition: neutral hBLM composed of cholesterol and phosphatidylcholine (PC:Chol), phosphatidylethanolamine-enriched hBLM (PC:Chol:PE), phosphatidylserine-enriched hBLM (PC:Chol:PS). b–f, ii Quantification of C2 fusion protein binding after wash-out. Data are presented as means ± standard error of the mean (n = 6–8 independent measurements). Means were compared by one-way ANOVA and post-hoc Tukey test. p values < 0.05 were considered as significant