Fig. 5
From: Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling
AAV-encoded C2 fusion probes label apoptotic cells in ex vivo organotypic hippocampal slices. a OHSC were transduced with AAVs carrying C2-SNAP, C2m2-SNAP, C2-mKate or C2m2-mKate at 5 days in vitro (5DIV) and maintained for 9 days for protein expression (n = 3 biological replicates). Apoptosis was induced by the treatment with 10 µM staurosporine for 16 h. Created by Biorender.com. b, c Confocal images of OHSC transduced with AAV-C2-mKate vs. AAV-C2m2-mKate or AAV-C2-SNAP vs. AAV-C2m2-SNAP. Cell nuclei were counterstained with DAPI. Scale bars 500 µm (whole slice) and 100 µm (enlarged area)