Fig. 6
From: Genetically encoded phosphatidylserine biosensor for in vitro, ex vivo and in vivo labelling
Application of adeno-associated viruses (AAV) for in vivo C2 probe delivery. At postnatal day 3 (P3) mice were injected with 1 × 1011 vg of AAV-C2-SNAP virus. Representative microscopy images of hippocampus after 2 weeks of AAV-C2-SNAP expression (P17) (n = 5 animals) and enlarged hippocampal areas. Cell nuclei were stained with DAPI, fused SNAP-tag was labelled with AlexaFluor647 (AF647). Control images represent BG-AF647 labelling of non-transduced brain tissue. Scale bar 250 µm (whole hippocampus) and 20 µm (enlarged area)