Fig. 1

KANK2, unlike KANK1, is localized within αVβ5 FAs. A Talin1 and talin2 are localized within integrin αVβ5 FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed, and stained with anti-talin1 antibody followed by Alexa-Fluor IgG1 555-conjugated antibody (red), anti-talin2 antibody followed by Alexa-Fluor IgG2b 488-conjugated antibody (green) and anti-integrin β5 antibody followed by Alexa-Fluor 647-conjugated antibody (magenta) and interference reflection microscopy (IRM) images were taken. B, C KANK2, unlike KANK1, localizes in talin1 and talin2-positive FAs. Forty-eight hours after seeding MDA-MB-435S cells were methanol fixed and stained with anti-talin1 or anti-talin2 antibody followed by Alexa-Fluor 546-conjugated antibody (red) or Alexa-Fluor 488-conjugated antibody (green) and anti-KANK1 or anti-KANK2 antibody followed by Alexa-Fluor 555-conjugated antibody or Alexa-Fluor 488-conjugated antibody (shown in magenta). Vinculin was visualized using conjugated anti-vinculin Alexa Fluor 647 antibody (shown in grey) and IRM images were taken. Analysis was performed using TCS SP8 Leica. Scale bar = 10 µm