Fig. 1

Rats experiencing sciatic nerve CCI exhibit mechanical and thermal hypersensitivities, inflammatory cytokine overproduction, and a cellular senescence-like response. A Experimental diagram illustrating sciatic nerve injury and behavior tests. B, C Mechanical allodynia and thermal hyperalgesia developed in the paws of the CCI rats and did not develop in the paws of the sham-operation rats from the first day after CCI until at least day 13 (n = 9). D Western blot images and quantification of IL-1α, IL-1β, TNF-α, IL-6, and IL-8 in the spinal cord after sham intervention or sciatic nerve CCI in rats (n = 3). E Representative photographs of SA-β-gal staining (the positive cells show a blue precipitate) with statistical results (n = 3, scale bars 100 μm). F Western blot images and quantification for senescence-related markers P16 and γ-H2AX in the spinal cord after sham operation or sciatic nerve CCI in rats (n = 3). **P < 0.01, ***P < 0.001