Fig. 3
From: Astrocyte senescence-like response related to peripheral nerve injury-induced neuropathic pain
Double staining and quantification of γ-H2AX in the spinal dorsal horn ipsilateral to sciatic nerve CCI or sham intervention. A Representative double-immunofluorescence staining images show the colocalization of γ-H2AX (green) with NeuN (neuronal marker)-, IBA1 (microglia marker)-, or GFAP (astrocyte marker)-positive cells (red). White arrows indicate colabelled cells. B Quantification analyses for the ratio of γ-H2AX immunoreactivity area to neuron/astrocyte/microglia marker immunoreactivity area in the spinal cord (n = 3, scale bars 100 μm). ***P < 0.001