Fig. 4
From: Astrocyte senescence-like response related to peripheral nerve injury-induced neuropathic pain
H2O2 and LPS induce senescence and inflammatory cytokine overproduction in primary cultures of spinal astrocytes. A Representative photographs of SA-β-gal staining (the positive cells contain a blue precipitate) and statistical results (n = 3, scale bars 200 μm). B Western blot images and quantification for senescence-related markers P16 and γ-H2AX in primary cultures of spinal astrocytes (n = 3). C Western blot images and quantification for IL-1α, IL-1β, TNF-α, IL-6, and IL-8 in primary cultures of spinal astrocytes (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001