Fig. 7

Dasatinib and quercetin inhibit senescence, alleviating CCI-induced pain, astrocyte senescence, and spinal cord inflammation. A, B Dasatinib (5 mg/kg) and quercetin (50 mg/kg) were gavaged to the CCI rats to detect mechanical allodynia and thermal hyperalgesia. The asterisks indicate the statistical significance of differences between the CCI + Veh and CCI + DQ groups (n = 9). C Representative photographs of SA-β-gal staining (the positive cells contain a blue precipitate) and statistical results (n = 3, scale bars 100 μm). D Western blot images and quantification for senescence-related markers P16 and γ-H2AX in the spinal cord of rats (n = 3). E Representative images of double staining and quantification for the coexpression of P16 (green) and GFAP, an astrocyte marker (red), in the spinal dorsal horn ipsilateral to sciatic nerve CCI or sham intervention (n = 3, scale bars 100 μm). F Western blot images and quantification of IL-1α, IL-1β, TNF-α, IL-6, and IL-8 in the spinal cord of rats (n = 3). *P < 0.05, **P < 0.01, ***P < 0.001