Fig. 2
From: N6-methyladenosine modification of PLOD2 causes spermatocyte damage in rats with varicocele
Functional analysis of differentially m6A methylated mRNAs between two groups. A An analysis of sequence motif among peak regions that contain m6A site in both control and VC groups. B Volcano plots showing differentially m6A-modified peaks in mRNAs based on |log2FC| > 1 and p-value < 0.05. In volcano plots, red blots represent hypermethylation and blue blots represent hypomethylation. C An analysis of the KEGG pathway of mRNAs that contain differentially methylated m6A sites. D MeRIP-qPCR analysis validates m6A enrichments for five hypermethylated genes and five hypomethylated genes. E RT-qPCR analysis validates mRNA expression levels for five upregulated genes and five downregulated genes. F According to the Integrative Genome Viewer (IGV) software, PLOD2 mRNA showed different m6A methylation patterns in the VC and control groups