Fig. 3

PLOD2 expression and m⁶A methylation level under oxidative stress in GC-2 cell. A Under oxidative stress, the mRNA expression levels of PLOD2 in GC-2 cell were detected using RT-qPCR. B Under oxidative stress, the m⁶A methylation levels of PLOD2 in GC-2 cell were detected using MeRIP-qPCR. C, D Western blot (C) and RT-qPCR (D) were used to measure PLOD2 protein and mRNA expression levels in GC-2 cells transfected with vector control or ALKBH5 construct for 24 h under oxidative stress. E MeRIP-qPCR analysis of PLOD2 m⁶A levels in control and overexpression of ALKBH5 in GC-2 cells under oxidative stress. F The mRNA levels of PLOD2 were checked for the indicated times in control and ALKBH5-overexpressed GC-2 cells after treatment with Act-D