Fig. 5

Targeting m⁶A demethylation of PLOD2 by CRISPR/dCas13b-ALKBH5 to regulate GC-2 cell proliferation and apoptosis under oxidative stress. A Figure depicts the position of the m⁶A site within PLOD2 mRNA and the target guide RNA’s target regions. B, C Under oxidative stress, m⁶A (B) and mRNA (C) levels of PLOD2 were measured in GC-2 cells transfected with dCas13b-ALKBH5 with a gRNA control or a gRNA for PLOD2. D GC-2 cells transfected for 24 h with dCas13b-ALKBH5 in combination with gRNA control or gRNA for PLOD2 are evaluated for cell proliferation using the CCK8 assay. E, F Transfection of GC-2 cells with dCas13b-ALKBH5 together with control gRNA or gRNA for PLOD2 to investigate cell apoptosis. G, H Under oxidative stress, GC-2 cells transfected with dCas13b-ALKBH5 combined with gRNA control or gRNA for PLOD2 for 24 h were examined for protein levels of PLOD2, p-AKT, Bax, and Bcl-2