Fig. 2

Characterization of cancer-associated adipocytes (CAAs). Adipocytes were cocultured with melanoma cells (lines: WM1341D, A375, WM9, and Hs294T) present on Transwell inserts, and then the expression of leptin, adiponectin, resistin, and FABP4 was established in obtained CAAs, as well as nondifferentiated 3T3L1 fibroblasts and control adipocytes, using real-time PCR analysis. For quantification, the level of expression was normalized to the expression of EEF2 and presented as the percent of control (untreated adipocytes). The mean of at least three biological repetitions ± standard deviation (SD) is shown. Asterisks indicate statistically significant differences between control adipocytes and 3T3L1/CAAs. The significance levels were set at p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), or p ≤ 0.0001 (****). FABP4 fatty acid-binding protein 4, EEF2 eukaryotic translation elongation factor 2