Fig. 7

Influence of melanoma cells on lactate secretion (A), ions (B, C), and glucose transport (D) by 3T3L1 cells, control adipocytes, and CAAs. CAAs were obtained from adipocytes cultured with melanoma cells (lines: WM1341D, A375, WM9, and Hs294T) present on Transwell inserts. A The level of secreted lactate was measured using a chemiluminescent reaction in a cell-conditioned medium devoid of serum, in which cells were grown for 24 h. The expression of NHE1 (B), MCT1 (C), and GLUT1 (D) was estimated through real-time PCR analysis. For quantification, the samples were normalized against the expression of EEF2. The mean of at least three independent repetitions ± SD is shown. Asterisks indicate statistically significant differences between control cells and 3T3L1/CAAs. The significance levels were set at p ≤ 0.05 (*), p ≤ 0.01 (**), p ≤ 0.001 (***), or p ≤ 0.0001 (****). NHE1 sodium/hydrogen exchanger 1, MCT1 monocarboxylate transporter 1, GLUT1 glucose transporter 1, EEF2 eukaryotic translation elongation factor 2