Fig. 3

CRISPR/Cas13d regulated the function of GZMA through controlling the expression of miR-25-3p and PAR1 in PARs pathway in chronic apical periodontitis. A, B The PAR1, TGF-β mRNA and protein expression levels were decreased significantly after inhibition of GZMA via crRNA/Cas13d. C, D The GZMA mRNA and protein expression levels were no changed after knockdown of TGF-β. E The miR-25-3p expression was promoted significantly after inhibition of GZMA. F, G After transfected with miR-25-3p inhibitor, the GZMA mRNA and protein expression levels were no changed. While, the TGF-β mRNA and protein expression levels were increased dramatically. H The expression of XPO5 was increased significantly. I The GZMA protein expression level was no changed after transfection of XPO5 siRNA. *Means P < 0.05, **means P < 0.01, ***means P < 0.001