Fig. 3

Western blot and immunohistochemical analyses of the neurons, astrocytes, and oligodendrocytes in different brain regions of the male offspring after prenatal LPS exposure. Representative western blots for the A neuronal markers MAP2, GFAP; semi-quantification of MAP2/GAPDH; B semi-quantification of GFAP/GAPDH, and C oligodendrocyte marker CNPase and semi-quantification of CNPase/GAPDH in the prefrontal cortex, striatum, and hippocampus tissues of the male offspring after prenatal exposure to 25 µg/kg LPS. Statistical analyses from three representative samples of western blot for the neuronal marker MAP2 are shown in the lower panel. Data are presented as means ± SEM. ** P < 0.01 versus PBS group by one-way ANOVA with the Tukey’s multiple comparisons test. D Representative images of immunohistochemical double staining for MAP2⁺ and GFAP⁺ cells in the prefrontal cortex, striatum and hippocampus tissues. GFAP⁺ cells were more abundant in the prefrontal cortex and hippocampus tissues of the male offspring after prenatal exposure to 25 µg/kg LPS on GD 9. E Representative images of immunohistochemical double staining for MAP2⁺ and O4⁺ cells in the prefrontal cortex, striatum, and hippocampus tissues. O4⁺ cells were more abundant in the prefrontal cortex and hippocampus tissues of the male offspring after prenatal exposure to 25 µg/kg LPS on GD 9. Scale bar, 250 µm. Arrows indicate GFAP⁺ or O4⁺ cell morphology