Fig. 5

Expression of toll-like receptor 2 (TLR2) and 4 (TLR4) during ESC-to-NSC transition and NSC-to-neural differentiation transition processes. A Schematic of the ESC-to-NSC transition process. Immunofluorescence staining for TLR2 and TLR4 expression in the differentiated cells on days 0 and 7 during ESC-to-NSC transition. Scale bar, 100 µm. B, C Quantification of TLR2⁺ and TLR4⁺ cells in ESCs and ESC-derived NSCs. Data are presented as means ± SEM (n ≥ 6). Significant difference and P values were analysed by one-way ANOVA with the Tukey’s multiple comparisons test. D Immunofluorescence staining for TLR2 and TLR4 in the differentiated MAP2⁺ cells on day 14 after NSC differentiation. Scale bar, 100 µm