Table 2 23 potential interacting partners of DSC1 identified using pull-down assay that can be modulated with parthenolide
Protein name | Protein description | UniProt ID | Gene name | Pull-down MCF7-DSC1-GFP vs. MCF7-GFP | Pull-down MCF7-DSC1-GFP + PTL vs. MCF7-DSC1-GFP | Total proteome MCF7-DSC1-GFP vs. MCF7-GFP | Total proteome MCF7-DSC1-GFP + PTL vs. MCF7-DSC1-GFP | ||||
|---|---|---|---|---|---|---|---|---|---|---|---|
log2 FC | q-value | log2 FC | q-value | log2 FC | q-value | log2 FC | q-value | ||||
DSG2 | Desmoglein-2 | Q14126 | DSG2 | 7.21 | 6.82E−53 | − 2.01 | 1.70E−52 | − 0.45 | 0.106 | 0.01 | 2.64E−04 |
CELR2 | Cadherin EGF LAG seven-pass G-type receptor 2 | Q9HCU4 | CELSR2 | 5.88 | 7.04E−53 | − 2.03 | 1.29E−45 | NA | NA | NA | NA |
PCDGD | Protocadherin gamma-B1 | Q9Y5G3 | PCDHGB1 | 5.22 | 8.02E−06 | − 1.32 | 1.60E−05 | NA | NA | NA | NA |
ALG11 | GDP-Man:Man(3)GlcNAc(2)-PP-Dol alpha-1,2-mannosyltransferase | Q2TAA5 | ALG11 | 5.15 | 4.75E−10 | − 2.24 | 2.35E−10 | NA | NA | NA | NA |
CADH1 | Cadherin-1 | P12830 | CDH1 | 5.11 | 1.20E−14 | − 1.39 | 1.28E−13 | − 0.02 | 1.79E−03 | − 0.09 | 0.224 |
CDIPT | CDP-diacylglycerol–inositol 3-phosphatidyltransferase | O14735 | CDIPT | 5.11 | 2.56E−09 | − 2.30 | 1.45E−06 | − 0.35 | 2.00E−03 | − 0.01 | 0.122 |
SATT | Neutral amino acid transporter A | P43007 | SLC1A4 | 3.86 | 5.27E−04 | − 2.41 | 4.09E−03 | NA | NA | NA | NA |
PCDGH | Protocadherin gamma-B5 | Q9Y5G0 | PCDHGB5 | 3.77 | 6.88E−04 | − 1.10 | 5.46E−04 | NA | NA | NA | NA |
PCDBD | Protocadherin beta-13 | Q9Y5F0 | PCDHB13 | 3.70 | 1.82E−06 | − 2.29 | 1.71E−03 | NA | NA | NA | NA |
TM165 | Transmembrane protein 165 | Q9HC07 | TMEM165 | 3.68 | 3.12E−03 | − 1.33 | 4.51E−03 | − 0.41 | 9.12E−06 | − 0.08 | 0.234 |
TIM23 | Mitochondrial import inner membrane translocase subunit Tim23 | O14925 | TIMM23 | 3.50 | 1.02E−03 | − 1.25 | 0.032 | 0.10 | 0.131 | − 0.03 | 0.244 |
UPK3L | Uroplakin-3b-like protein 1 | B0FP48 | UPK3BL1 | 3.39 | 1.13E−10 | − 1.08 | 5.46E−04 | NA | NA | NA | NA |
TNPO1 | Transportin-1 | Q92973 | TNPO1 | 3.18 | 8.96E−06 | − 1.16 | 4.33E−03 | 0.02 | 0.139 | 0.17 | 9.29E−03 |
FAT1 | Protocadherin Fat 1 | Q14517 | FAT1 | 2.96 | 1.58E−14 | − 1.98 | 4.15E−12 | NA | NA | NA | NA |
CADH3 | Cadherin-3 | P22223 | CDH3 | 2.89 | 5.37E−06 | − 2.21 | 2.58E−03 | NA | NA | NA | NA |
SFXN4 | Sideroflexin-4 | Q6P4A7 | SFXN4 | 2.78 | 2.14E−03 | − 2.96 | 3.17E−03 | NA | NA | NA | NA |
RAB13 | Ras-related protein Rab-13 | P51153 | RAB13 | 2.58 | 3.25E−03 | − 1.94 | 0.026 | 0.04 | 0.175 | − 0.16 | 0.154 |
MOT1 | Monocarboxylate transporter 1 | P53985 | SLC16A1 | 2.24 | 6.75E−03 | − 1.39 | 7.07E−03 | − 0.71 | 2.77E−03 | 0.22 | 0.092 |
TIDC1 | Complex I assembly factor TIMMDC1, mitochondrial | Q9NPL8 | TIMMDC1 | 2.13 | 3.06E−04 | − 1.37 | 4.51E−03 | − 0.43 | 8.16E−03 | − 0.06 | 0.197 |
ERBB3 | Receptor tyrosine-protein kinase erbB-3 | P21860 | ERBB3 | 1.68 | 0.031 | − 2.12 | 5.94E−03 | NA | NA | NA | NA |
RS23 | 40S ribosomal protein S23 | P62266 | RPS23 | 1.61 | 0.019 | − 1.53 | 0.020 | − 0.09 | 0.107 | − 0.19 | 0.253 |
RT12 | 28S ribosomal protein S12, mitochondrial | O15235 | MRPS12 | 1.56 | 8.30E−03 | − 1.53 | 0.011 | NA | NA | NA | NA |
TBAL3 | Tubulin alpha chain-like 3 | A6NHL2 | TUBAL3 | 1.32 | 0.047 | − 1.82 | 0.045 | − 0.23 | 0.166 | − 0.34 | 0.056 |