Fig. 3

LPS outcomes on programmed cell mechanisms. A Representative images and B FACS detection of pyroptosis. C Intracellular NLRP3 levels assessed by FACS analysis. Representative images and cytofluorimetric evaluation of D, E lysosomes, F, G autophagy, and H immunoblotting of LC3B II/I. I Representative images and J FACS-detected mitochondrial ROS levels. K Representative annexin V-FITC and PI-staining detected by FACS analysis. Mean ± SD, n = 3. M, molecular weight markers; lane 1, Ctr; lane 2, LPS. Scale bars = 100 μm. ^‡p < 0.01 versus Ctr, ^¶p < 0.001 versus Ctr. Statistical analysis of data was performed using Student’s t-tests