Fig. 4

i-miR-15b opposed the LPS-related inflammation. A Hsa-miR-15b-5p expression measured by qRT–PCR in TeloHAEC transfected with antagomir negative control (NC) or antagomiR hsa-miR-15b-5p (i-miR-15b). Detection of B LDH, C NO, D VCAM1, E ICAM1, F MCP-1, G IL-1β, and H IL-18 levels. Assessment of caspase-4 I activity and J levels. SIRT4 levels assessed by K ELISA assay and L immunoblotting. M Representative images and N FACS analysis of pyroptosis. O Intracellular NLRP3 levels assessed by FACS detection. Mean ± SD, n = 3. M, molecular weight markers; lane 1, Ctr; lane 2, NC; lane 3, NC + LPS; lane 4, i-miR-15b; lane 5, i-miR-15b + LPS. Scale bars = 100 μm. *p < 0.05 versus NC, ^‡p < 0.01 versus NC, ^§p < 0.001 versus NC, ^•p < 0.05 versus NC + LPS, ^†p < 0.01 versus NC + LPS. Statistical analysis of data was performed using one-way ANOVAs.