Fig. 5

i-miR-15b counteracted the LPS-induced programmed mechanisms. Representative images and cytofluorimetric graph representation of A, B lysosomes, C, D autophagy, and E immunoblotting of LC3B II/I. F, G Representative images and graph of mitochondrial ROS and H annexin V-FITC and PI-staining detected by FACS analysis. Mean ± SD, n = 3. M, molecular weight markers; lane 1,  Ctr; lane 2, NC; lane 3, NC + LPS; lane 4, i-miR-15b; lane 5, i-miR-15b + LPS. Scale bars = 100 μm. ^‡p < 0.01 versus NC, ^§p < 0.001 versus NC, ^†p < 0.01 versus NC + LPS. Statistical analysis of data was performed using one-way ANOVAs.