Fig. 7

i-PCSK9 and i-miR-15b prevented LPS-related dysfunction. Representative images and graphical representation of FACS analysis of A, B pyroptosis and C, D autophagy. E Representative immunoblotting of SIRT4. Mean ± SD, n = 3. M, molecular weight markers; lane 1, Ctr; lane 2,  NC; lane 3,  NC + i-PCSK9; lane 4,  NC + LPS; lane 5,  NC + i-PCSK9 + LPS; lane 6, i-miR-15b; lane 7, i-miR-15b + i-PCSK9; lane 8, i-miR-15b + LPS; lane 9, i-miR-15b + i-PCSK9 + LPS. Scale bars = 100 μm. F The relative luciferase activity in EC co-transfected with luciferase reporter plasmid containing the SIRT4 3′-UTR sequence and with antagomir, NC or i-miR-15. *p < 0.05 versus NC, ^‡p < 0.01 versus NC, ^¶p < 0.001 versus Ctr, ^§p < 0.001 versus NC, ^•p < 0.05 versus NC + LPS, ^†p < 0.01 versus NC + LPS, ^°p < 0.05 versus i-miR-15b + LPS. Statistical analysis of data was performed using one-way ANOVAs.