Fig. 1

Schematics of the domain organization of Cas proteins and crystal structures of Cas protein-sgRNA-target sequence complex. A SpCas9 (PDB: 4OO8) (B) FnCas12a (PDB: 6I1K) (C) LbuCas13a (PDB: 5WXP). REC recognition lobe, NUC nuclease lobe, BH bridge helix, Arg arginine-rich bridge helix, CTD C-terminal domain, WED Wedge, PI protospacer adjacent motif (PAM) interacting, NTD N-terminal domain, HEPN higher eukaryotes and prokaryotes nucleotide-binding domains