Fig. 3

MCCC2 deficiency led to telomere shortening. A Reactive oxygen species (ROS) amounts were detected by flow cytometry in oeMCCC2, KO cells, and their controls respectively. B Mitochondrial membrane potential in different cells was evaluated by flow cytometry with JC-1 staining method. C oeMCCC2, KD, KO cells, and their controls were cultured for the indicated population doublings (PDs) and then subjected to the telomere restriction fragment (TRF) analysis. The length of telomere were calculated based on the phosphor imaging by TeloRun software. D Relative mRNA level of hTERT was quantified by RT-qPCR. E The relative telomerase activity in oeMCCC2, KD, KO cells, and their controls were quantified by Q-TRAP assay. Paired two-tailed Student’s t-test was used to calculate p value. All data presented as mean ± SEM (n = 3). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001