Fig. 4

MCCC2 formed a complex with the shelter in protein. A Co-immunoprecipitation (Co-IP) experiment was performed by applying the anti-TRF2 antibody from MCCC2-FLAG expressing cell extract, and IgG served as a negative control. The result showed that endogenous TRF2 formed a complex with exogenous MCCC2. Star indicates unspecific band nearby the target band. B Co-IP experiment was performed by applying the anti-FLAG antibody from MCCC2-FLAG expressing nucleus extract with the nucleus and cytoplasm separated kit. The result showed that exogenous MCCC2 interacted with endogenous TRF2. Star indicates unspecific band nearby the target band. C Scheme of the human MCCC2 protein motifs: 2–22, Mitochondrial targeting signals; 343–372, Acyl-CoA binding region. ∆2–22 and ∆343–372 indicate two individual construct with the corresponding motif deleted. D MCCC2 KO cells were stably transfected with FLAG-Tagged full-length and FLAG-Tagged mutants (∆2–22 or/and ∆343–372) of MCCC2 plasmids. Western blotting analysis suggested that they were all able to express at a similar level in the cells. E Cell lysates from (D) were subjected to Co-IP experiment by applying the anti-TRF2 antibody. The result showed that endogenous TRF2 formed a complex with mutant MCCC2