Fig. 5

MCCC2 was upregulated in CRC and associated with longer OS. A The mRNA level of MCCC2 was significant higher in CRC tissues than in peritumor tissues in the TCGA cohorts (n = 50). Paired two-tailed Student’s t-test was used to calculate p value, ***p < 0.001. B The mRNA levels of MCCC2 in primary tumors, matched peritumor, metastatic tumor, and normal tissues of CRC patients (n = 41) were quantified by RT-qPCR and compared. Paired two-tailed Student’s t-test was used to calculate p value,*p < 0.05, **p < 0.01. C Representative image of IHC staining of MCCC2 in CRC tissue and adjacent tissue were showed. Scale bars: 200 µm. D IHC scores for MCCC2 in a tissue microarray of CRC patients (n = 210) were analyzed. Paired two-tailed Student’s t-test was used to calculate p value, ****p < 0.0001. E Kaplan–Meier survival curves of overall survival based on IHC scores of MCCC2 in the tissue microarray. The MCCC2 protein high expression group was associated with longer OS than the MCCC2 protein low expression group (p = 0.01). F Survival curves based on TCGA database showed that patients with high MCCC2 mRNA expression survived significantly longer than those with low MCCC2 mRNA expression (p = 0.0016). G Survival curves based on GEO41258 datasets showed that patients with high MCCC2 mRNA expression survived significantly longer than those with low MCCC2 mRNA expression (p = 0.0009). *Data collection only included patients with carcinoma in situ