Fig. 6
MCCC2 KD inhibited CRC cell proliferation in vivo. A 1 × 106 of HCT116 cells with MCCC2 stably KD and its control cells were injected into nude mice subcutaneously (n = 6 per group). Tumors were harvested on day 11. The weights of tumors formed from KD group were lower than the control group. Paired two-tailed Student’s t-test was used to calculate p value. Data represent the mean ± SEM (n = 6). *p < 0.05. B Sectioned tumor samples from animals were subjected to H&E staining, IHC staining with anti-MCCC2 and anti-Ki-67 antibody, and representative images are showed. C Quantification of Ki-67 staining in (C). Paired two-tailed Student’s t-test was used to calculate p value. Data represent the mean ± SEM (n = 3). ****p < 0.0001. D Integrative diagram for MCCC2 in mitochondria regulation, telomere maintenance, and CRC cellular behaviors. MCCC2 inhibited the expression of MFN1/MFN2/OPA1, which in turn inhibited mitochondria fission that is beneficial for cellular energy production; MCCC2 could also form a complex with TRF2, playing potential role in telomere maintenance. Together, MCCC2 expression promoted CRC proliferation, invasion, and migration