Fig. 1

lnc-Helf is increased in mouse and human hepatic fibrosis. A, B The expression of lnc-Helf and Acta2 (α-SMA) were analyzed by qRT–PCR in livers from mice that underwent CCl₄ treatment or mice that underwent BDL for different timepoints. C The PCR products from the 5'-RACE procedure and 3'-RACE procedure underwent agarose gel electrophoresis. D The nuclei or cytoplasm of single-cell suspensions from the normal liver were isolated, and qRT–PCR analysis detected the expression of lnc-Helf, Neat1, and Gapdh. E qRT–PCR was used to assess the expression of lnc-Helf in HCs, HSCs, HMs, and LSECs that were isolated from livers of mice with or without CCl₄ treatment for 6 weeks. F qRT–PCR was used to assess the expression of lnc-Helf and Acta2 in the HSCs after culture-induced activation for indicated times. G qRT–PCR was used to assess the expression of lnc-HELF in liver samples from fibrotic patients (n = 28) and healthy people (n = 6). Data are presented as mean ± SEM. *p < 0.05, unpaired Student’s t test (A, B, and E–G)