Fig. 7

lnc-Helf promotes hepatic inflammation and fibrosis by interacting with PTBP1. A In vitro RNA pulldown–mass spectrometry assay in single liver cell lysates. B The specific interaction of sense lnc-Helf RNA with PTBP1 protein was determined by western blot. C, D RIP–qRT–PCR assay in single-cell suspensions from the liver (C) and RAW264.7 cells (D). E qRT–PCR was used to detect the expression of lnc-Helf, Ptbp1, Tnf-α, Mcp-1, Il-1β, Il-6, Pcna, Cyclin D1, and Cyclin E1 in lnc-Helf-overexpressed RAW264.7 cells simultaneously transfected with siPTBP1. F Mature supernatant IL-1β levels in lnc-Helf-overexpressed RAW264.7 cells simultaneously transfected with siPTBP1 was detected by ELISA. G Cell proliferation of lnc-Helf-overexpressed RAW264.7 cells simultaneously transfected with siPTBP1 was detected by CCK8. H The expression of lnc-Helf, Ptbp1, Acta2, Col1α2, Timp1, Pcna, and Cyclin E in lnc-Helf-upregulated primary HSCs, simultaneously transfected with siPTBP1, was detected by qRT–PCR. Data are presented as mean ± SEM. ^*/#p < 0.05. *p < 0.05 for IgG RIP or pcDNA3.1 + si-NC or LV-control + si-NC. ^#p < 0.05 for pcDNA3.1-lnc-Helf + si-NC or LV-lnc-Helf + si-NC, unpaired Student’s t test (C, D) and one-way ANOVA (E–H)