Fig. 2

MIR4435-2HG promotes the progression of HCC cells in vitro and in vivo. A MIR4435-2HG expression in HCC cells with lentivirus infection. B Cellular proliferation of HCC cells with MIR4435-2HG overexpression or control was detected by CCK8 assay. C Cellular proliferation of HCC cells with MIR4435-2HG overexpression or control was detected by EdU assay. D Colony formation ability of HCC cells with MIR4435-2HG overexpressed or control was detected. E Tumor volume grow curves of subcutaneous tumor model. F Weight of subcutaneous tumors formed by Huh7 cell with MIR4435-2HG overexpression or control. G Photograph of subcutaneous tumors formed by Huh7 cell with MIR4435-2HG overexpression or control. H Representative images of Ki-67 and PCNA immunohistochemical staining in tumor tissues. The data are shown as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Scale bar, 50 μm