Fig. 3

MIR4435-2HG enhances the stemness properties of HCC cells in vitro and in vivo. A Expression of stemness markers in HCC cells with MIR4435-2HG overexpression or control was detected by qPCR. B Expression of stemness markers in HCC cells with MIR4435-2HG overexpression or control was detected by western blot. C The spheroids form ability of HCC cells with MIR4435-2HG overexpression. D The spheroids form ability of HCC cells with MIR4435-2HG interference. E Overexpression of MIR4435-2HG could significantly enhance the sphere-initiating cell frequency in HCC cell in vitro. F Overexpression of MIR4435-2HG could significantly enhance the sphere-initiating cell frequency in HCC cell in vivo. The data are shown as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001