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Fig. 4 | Cellular & Molecular Biology Letters

Fig. 4

From: N6-methyladenosine-modified oncofetal lncRNA MIR4435-2HG contributed to stemness features of hepatocellular carcinoma cells by regulating rRNA 2′-O methylation

Fig. 4

MIR4435-2HG directly binds to NOP58 and IGF2BP1. A The silver-stained SDS-PAGE gel of proteins pulled down by MIR4435-2HG sense and antisense RNAs. B Western blot analysis showed that NOP58 and IGF2BP1 were in the proteins pulled down by MIR4435-2HG. C RIP-PCR assay was performed to enrich MIR4435-2HC by antibody against NOP58 or IGF2BP1 in extracts from HCC cells. D The predicted secondary structure of MIR4435-2HG. E Serial deletions of MIR4435-2HG were constructed to identify the specific association of MIR4435-2HG and NOP58 or IGF2BP1

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