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Fig. 5 | Cellular & Molecular Biology Letters

Fig. 5

From: N6-methyladenosine-modified oncofetal lncRNA MIR4435-2HG contributed to stemness features of hepatocellular carcinoma cells by regulating rRNA 2′-O methylation

Fig. 5

IGF2BP1 regulates MIR4435-2HG expression in a m6A dependent way. A Knockdown of IGF2BP1 by siRNA in HCC cells. B The expression of MIR4435-2HG in HCC cells with IGF2BP1 knockdown. C The relationship between MIR4435-2HG expression and IGF2BP1 expression in 20 HCC samples. D The predicted conserved m6A modification sites in MIR4435-2HG. E MeRIP-PCR analysis was performed to confirm the enrichment of m6A modification in MIR4435-2HG in HCC cells. F Knockdown of METTL3 by siRNA in HCC cells. G The enrichment of m6A modification in MIR4435-2HG in HCC cells with METTL3 knockdown. H The expression of MIR4435-2HG in HCC cells with METTL3 knockdown. I The dual-luciferase reporter assay showed that METTL3 and IGF2BP1 regulated MIR4435-2HG expression in a m6A dependent. The data are shown as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001

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Cellular & Molecular Biology Letters

ISSN: 1689-1392

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