Fig. 6

NOP58 was stabilized by MIR4435-2HG and mediated the pro-tumorigenetic role of MIR4435-2HG in HCC. A The expression of NOP58 mRNA in HCC cells with MIR4435-2HG interference in HCC cells. B The expression of NOP58 protein in HCC cells with MIR4435-2HG overexpressed in HCC cells. C Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of CHX. D Western blot analysis detected the expression of NOP58 protein in HCC cells with treatment of MG132. E Expression analysis of NOP58 protein in HCC based on CPTAC database. F Representative images of the expression of NOP58 protein in paired tumor and nontumor tissues by IHC. G The relationship between MIR4435-2HG expression and NOP58 expression in 20 HCC samples. H Knockdown of NOP58 by siRNA in HCC cells. I Cellular proliferation of HCC cells was detected by CCK8 assay. J Cellular proliferation of HCC cells was detected by EdU assay. K Colony formation ability of HCC cells was detected. L The spheroids form ability of HCC cells. M Expression of stem cell markers were detected in the indicated groups by qPCR. The data are shown as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. White scale bar, 100 μm