Fig. 7

Overexpression of MIR4435-2HG increases rRNA 2′f-O methylation and IRES-dependent translation of oncogene in HCC. A RTL-P assay was conducted to detect the 2’-O methylation level of rRNA in HCC cells with MIR4435-2HG overexpression or NOP58 knockdown. B OP-Puro incorporation assay was conducted to detect the protein synthesis rate. C The bi-cistronic luciferase reporter was constructed as above and the Poliovirus (PV) IRES activity was calculated as the ratio of firefly luciferase activity over renilla luciferase activity. D The bi-cistronic luciferase reporter was constructed as above and the IRES-dependent translation (Fluc/Rluc) from IRES elements of MYC and IGF1R was measured. E qPCR analysis detected the expression of MYC and IGF1R mRNA in the indicated groups. F The protein expression of MYC and IGF1R in the indicated groups was detected by western blot analysis. G Model of m6A modified MIR4435-2HG regulating stemness maintenance of HCC by stabilizing NOP58. The data are shown as mean ± SD. ^*P < 0.05, ^**P < 0.01, ^***P < 0.001. Scale bar, 100 μm