Fig. 4

CEBPB induces the transcription of BZW1. A Venn diagram of the regulatory downstream targets of CEBPB downloaded from the hTFtarget database and the differentially expressed genes in the GSE157293 and GSE153547 datasets. B Analysis of single-cell expression of BZW1 in the kidney by the Human Protein Atlas database. C BZW1 expression in kidney tissues of patients with LN (n = 23) was analyzed using RT–qPCR. D Correlation between BZW1 and CEBPB expression in kidney tissues of patients with LN (n = 23). E BZW1 expression in kidney tissues of mice was examined using RT–qPCR. F BZW1 was predicted as a downstream transcriptional regulatory target of CEBPB using hTFtarget. G CEBPB binding sites on BZW1 promoter predicted using the Jaspar database. H The binding relation between CEBPB and BZW1 promoter using ChIP–qPCR. I CEBPB and BZW1 expression in BMDM with knockdown of CEBPB by RT–qPCR. J The transcriptional regulation between CEBPB and BZW1 by dual-luciferase reporter assay. Data are presented as means ± SD and representative of three independent experiments (n = 8, *p < 0.05). Data were statistically analyzed using an unpaired t-test (C, H), one-way ANOVA (E, J), or two-way ANOVA (I)