Fig. 6

Overexpression of BZW1 overturns the mitigating effects of sh-CEBPB on LN in mice. MRL/lpr mice were further treated with sh-CEBPB + oe-NC or sh-CEBPB + oe-BZW1. A The protein expression of BZW1 in mouse kidney tissues was measured using western blot assays. B Expression of BZW1 in mouse kidney macrophages detected by dual-label immunofluorescence. C The creatinine levels in the serum of mice. D The ratio of urine protein to creatinine in mice. E The relative content of dsDNA in the serum of mice. F The IgG deposition in the kidney of mice. G C3 content in the serum of mice. H Histopathological analysis of kidney tissues of mice by HE staining. I PAS-positive deposition in the kidney tissues of mice. J Immunohistochemical detection of CD68 and F4/80 in the kidney tissues of mice. K CD11b⁺CD68⁺ macrophages in single cell suspensions of mouse kidney tissues analyzed by flow cytometry. L Expression of IL-1β, IL-6, and TNF-α in kidney tissues of mice was measured using ELISA. Data are presented as means ± SD (n = 8, *p < 0.05). Data were statistically analyzed using an unpaired t-test (A, C–I, K–L) or two-way ANOVA (J)