Fig. 7

BZW1 phosphorylates eIF2α to promote macrophage glycolysis and ER stress. BMDM induced with Ova-IC were further treated with sh-CEBPB + oe-NC or sh-CEBPB + oe-BZW1. A The total protein level and phosphorylation levels of eIF2α in Ova-IC-treated BMDM. B The expression of ER stress-related proteins CHOP and GRP78 in Ova-IC-treated BMDM. C The protein expression of p-eIF2α, eIF2α, CHOP, and GRP78 expression in BMDM. D ECAR and OCR traces for BMDM. E The ECAR/OCR ratio in BMDM. F The glucose uptake capacity of BMDM was measured using a glucose uptake assay. G Glycolysis-related protein HK2 and LDHA expression in BMDM were measured using western blot. H Expression of IL-1β, IL-6, and TNF-α in BMDM by RT–qPCR. I Expression of IL-1β, IL-6, and TNF-α in BMDM in response to 4-PBA by RT–qPCR. Data are presented as means ± SD and representative of three independent experiments (*p < 0.05). Data were statistically analyzed using an unpaired t-test (E, F) or two-way ANOVA (A–D, G–I)